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Which High-Fidelity polymerase is better?

PCR High-Fidelity Polymerase

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2 replies to this topic

#1 Guf

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Posted 08 March 2013 - 04:25 AM

Hello,

I need to amplify 7 kb sequence with high fidelity. We have three high-fidelity polymerases in our lab:


PfuUltra High-Fidelity DNA polymerase (from QuikChange II site directed mutagenesis kit, Agilent)

KAPA Hifi HotStart DNA polymerase (Kapabiosystems)

Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs)


Naturally, each manufacturer claims that his product is the best one. I have no experiences with HF polymerases so would it be possible to advise me which polymerase is really the best, please? Thank you.

#2 phage434

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Posted 08 March 2013 - 08:53 AM

My go-to polymerase is Q5. PfuUltra is somewhat tricky, no experience with Kappa.

#3 bigudukaz

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Posted 05 April 2013 - 12:42 AM

Kapa is no good compared to the other ones. best options - Q5 (NE biolabs) and Phusion high fidelity DNA polimerase (from ThermoFisher) they are both very good, but it depends on the system of the reaction which one is better. but for 7kb i guess Phusion is better.





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