RNA gel electrophoresis for integrity check - denaturing or non-denaturing??RNA gel electrophoresis denaturing gels formaldehyde gel RNA integrity RNA gel
Posted 07 March 2013 - 10:12 PM
I would like to know which is the preferred method for checking the integrity of RNA (stored for >1 year in -80) using gel electrophoresis. I want to use this RNA for real-time PCR. Should I do denaturing gels or non-denaturing gels?
In non-denaturing gels. should I use 1x TAE or 1x TBE?
Thanks a lot,
Posted 07 March 2013 - 11:30 PM
Posted 07 March 2013 - 11:37 PM
Posted 11 March 2013 - 11:08 PM
Posted 12 March 2013 - 01:22 AM
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