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Question regarding yeast strain with ura3-52 mutation

Yeast selectable marker uracil auxotroph ura3-52

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#1 Daniel0306

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Posted 07 March 2013 - 02:41 PM

I am working with a yeast strain that contains (among other mutations/deletions) a ura3-52 mutation which should result in a uracil auxotroph. However, these cells seem to grow very well in synthetic yeast medium lacking uracil. Does anyone have an explanation for this? Thanks.

#2 HOYAJM

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Posted 20 March 2013 - 10:13 AM

The easiest explanation would be there is contamination. I would re-streak to isolation and try again. If this was a stock culture it could have gotten contaminated along the way. The only other explanation would be that the mutation is not present.





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