I am working with a yeast strain that contains (among other mutations/deletions) a ura3-52 mutation which should result in a uracil auxotroph. However, these cells seem to grow very well in synthetic yeast medium lacking uracil. Does anyone have an explanation for this? Thanks.
0
Question regarding yeast strain with ura3-52 mutation
Started by Daniel0306, Mar 07 2013 02:41 PM
Yeast selectable marker uracil auxotroph ura3-52
1 reply to this topic
#2
HOYAJM
-
- Active Members
-
- 121 posts
Veteran
8
Neutral
Neutral
Posted 20 March 2013 - 10:13 AM
The easiest explanation would be there is contamination. I would re-streak to isolation and try again. If this was a stock culture it could have gotten contaminated along the way. The only other explanation would be that the mutation is not present.
Also tagged with one or more of these keywords: Yeast, selectable marker, uracil auxotroph, ura3-52
 |
Protocols and Techniques Forums →
Microbiology →
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
contamination of human cell lines with yeastStarted by Guest_perunica_* , 11 Dec 2012  saccharomyces, yeast
|
|
|
||
Protocols and Techniques Forums →
Microbiology →
Alcohol productionStarted by Guest_twood_* , 23 Nov 2012 Yeast, Alchohol
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
|
|
|
||
Protocols and Techniques Forums →
DNA Methylation and Epigenetics →
|
|
|
