I am working with a yeast strain that contains (among other mutations/deletions) a ura3-52 mutation which should result in a uracil auxotroph. However, these cells seem to grow very well in synthetic yeast medium lacking uracil. Does anyone have an explanation for this? Thanks.
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Question regarding yeast strain with ura3-52 mutation
Started by Daniel0306, Mar 07 2013 02:41 PM
Yeast selectable marker uracil auxotroph ura3-52
1 reply to this topic
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HOYAJM
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Posted 20 March 2013 - 10:13 AM
The easiest explanation would be there is contamination. I would re-streak to isolation and try again. If this was a stock culture it could have gotten contaminated along the way. The only other explanation would be that the mutation is not present.
Also tagged with one or more of these keywords: Yeast, selectable marker, uracil auxotroph, ura3-52
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