Lets say I wanted to clone a region of DNA (a 2.0 kB putative promoter region) from genomic DNA into a reporter vector, like pGL3 basic, by PCR-mediated amplification.
Should I use a high fidelity polymerase for the PCR or just regular Taq?
If I use a high fidelity polymerase, which one should I use?
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4 replies to this topic
#2
GNANA
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Posted 06 March 2013 - 09:40 AM
always better to go with high fidelity pol for cloning. there are lot available in the market, like Phusion (Neb), platinum pfx , Accuprime Pfx (invitrogen), Pfuultra (stratagene) etc ....i would go with Accuprime, as i am personally used to that.
I would prefer being perfectionist rather than a passionist in Research.
I always had an alternate hypothesis....
I always had an alternate hypothesis....
#3
bob1
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Thelymitra pulchella
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Posted 06 March 2013 - 11:47 AM
I like KOD, but the pfu range tends to be the standard.
#4
srpres
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Posted 06 March 2013 - 12:43 PM
bob1, on 06 March 2013 - 11:47 AM, said:
I like KOD, but the pfu range tends to be the standard.
#5
bob1
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Posted 06 March 2013 - 12:56 PM
KOD is KOD polymerase
pfu range means any one of the DNA pols that are derived from pfu (e.g. pfuUltra).
pfu range means any one of the DNA pols that are derived from pfu (e.g. pfuUltra).
