Posted 05 March 2013 - 11:21 PM
Everybody, i need your help.
I buy Trypan blue dye 60% (302643) from sigma.
Then I need to use it in cell culture. Usually people use trypan blue solution 0.4% .
How I can prepare that from Trypan blue dye 60% (302643)?
Thank you very much
Posted 08 March 2013 - 06:26 AM
i asked so many questions before this
this is my 3rd account since the other 2 one got problem so i cant longer used the account
I asked this because, at my place, no one use that trypan blue powder.
all of them used the ready made one, so I think the best is to ask people in Bioforum.
Thank you so much
I bought 2 bottle and it must be used, rather than waste the money
Posted 19 December 2013 - 02:11 PM
What about a control? We work in a clinical lab and use TB to get a cell vialbilty of the bone marrow products after collection, before infusion etc. We are looking for a way to have controls to prove TB is working....perferably something we can make that kills half the cells so we can see both positive and negative. Any ideas?