4 replies to this topic

[xxcici]

Hi guys, I want to ask about one question of the interaction between virus and cell surface receptor. Virus need to bind to the receptor for attachment. After attachment, virus will uncoat outside of the cell or inside the cell? Then, in vitro, if I use the cell surface receptor chemical composition to attract virus, will the virus uncoat itself to release genome?

[leelee]

Every virus is different. They all target different receptors and are internalised by different mechanisms (or multiple mechanisms). Perhaps if you give more specific details, someone can help you?

[bob1]

The only thing I would add is that the virus uncoats inside the cell - otherwise the stuff inside the virus will not be replicated...

[xxcici]

The virus is norovirus and its receptor HBGA carbohydrates, hepatitis A virus and HAVcr-1 (glycophorin).

[bob1]

Human norovirus won't grow in culture, but you can grow murine.  I don't think using the receptor to bind the virus itself is enough to get it to uncoat, there should be other signals too.  Have a look in Field's Virology, there should be a starting point somewhere in there.