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a question about Immunoprecipitation


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#1 Beatrice

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Posted 03 February 2004 - 06:22 AM

Hello, everyone!
My question is: does C-Abl kinase expressed in vivo (in HEK293 cells), binds to protein A-sepharose during the immunoprecipitation?
Thanks a lot !

Edited by Beatrice, 03 February 2004 - 06:51 AM.


#2 Shubenok

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Posted 03 February 2004 - 10:19 AM

Protein A specifically bind CH3( or CH2 i don`t remember exactly) domain of Ab or in original cases Vh domain so that....




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