My question is: does C-Abl kinase expressed in vivo (in HEK293 cells), binds to protein A-sepharose during the immunoprecipitation?
Thanks a lot !
Edited by Beatrice, 03 February 2004 - 06:51 AM.
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#1
Beatrice
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Posted 03 February 2004 - 06:22 AM
Hello, everyone!
My question is: does C-Abl kinase expressed in vivo (in HEK293 cells), binds to protein A-sepharose during the immunoprecipitation?
Thanks a lot !
My question is: does C-Abl kinase expressed in vivo (in HEK293 cells), binds to protein A-sepharose during the immunoprecipitation?
Thanks a lot !
#2
Shubenok
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Posted 03 February 2004 - 10:19 AM
Protein A specifically bind CH3( or CH2 i don`t remember exactly) domain of Ab or in original cases Vh domain so that....
