A quick cloning question:
I want to delete part of a plasmid. I double digest with to REs which result in sticky ends. I gel-purify the desired linearized fragment. I blunt-end with T4 polymerase and the ligate the ends together by T4 ligase.
Before the ligation, do I need to use T4 kinase to add phosphate groups to 5' ends?
thank you
-k
0
ligating blunt ends of a linearized plasmid, is kinase necessary?
Started by kaveh, Mar 04 2013 11:52 AM
1 reply to this topic
