2 replies to this topic

[biolabs]

Hi everyone,

I've been reading all your posts for the past few weeks and now have the courage to register and post.     I am in need of your help.  I need to design an experiment to figure out the genomic structure of the insulin gene.  Could anyone please point me in the right direction?

Thanks!

Arielle

[green]

Hi Arielle,

There are lots of paper reporting how to determine genomic structure of genes and there are different strategies too. So just search medline using the query ((genomic[Title] AND structure[Title]) AND gene[Title]) and you will get a bounch of papers on this topic. Try to understand what strategies others use and choose one you think is feasible for you. If your gene is a know gene, try using the genome database to see if the genomic structure is already there. The places you can try are : Ensembl at http://www.ensembl.org, UCSC at http://genome.ucsc.edu or NCBI AceView at http://www.ncbi.nlm....mbly/index.html.

Hope it's useful.

[biolabs]

Thanks!