hi everyone,
i am trying to create a stable transfection in MDA-MB-231 cells with G418. I haven't done LC50 with G418 on MDA-MB-231, but i thought i would ask what would be a good starting concentration. I have read that 1500mcg/ml is appropriate, but it seems quite high. what do you guys think?
my best,
jeff kwak
0
creating stable line for MDA-MB-231
Started by jeff kwak, Mar 03 2013 01:01 PM
G418 stable transfection MDA-MB-231
1 reply to this topic
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 03 March 2013 - 03:14 PM
What you should do is have a look through the literature, there is bound to be someone who has done this before. You should then take the value you found and do a titration above and below that value to see if it works for your particular cells.
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