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Whole blood sample (ex vivo) stimulation for downstream RNA-assays

cell culture rna igf1 stimulation

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#1 dude3005

dude3005

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Posted 03 March 2013 - 12:34 AM

We plan to investigate the influence of growth factors on the bloods' transcriptome. Instead of doing stimulation assays in cell culture, I was faced with the idea to directly stimulate whole blood samples ex vivo (as described for some cytokine assays). The argument is that this is the only way to identify common RNA-signatures influenced by growth factor stimulation.


While I do have my own opinion towards this assay I just want to collect pro's and con's for this procedure.



Best, D.







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