1 reply to this topic

[freakyboy]

Hi,

I am new to the PCR. Now I dealing with the colony PCR, I have a problem which prevails like a stubborn thing. I working with gram negative bacteria which lacks in peptidoglycan and it is easy to extract the DNA from those. My problem is, I tried the template preparation by the usual method. But, I getting the template DNA sometimes luckily and for the most of times it isn’t working. I tried the other methods of template preparation by using lysis buffer.  Even though the problem persists.

Can anyone solve this issue?

Thanks in advance

[Curtis]

we already have these guidelines at protocol-online.org but this video might also be helpful. I follow this method too.