Posted 28 February 2013 - 10:15 AM
Wikipedia (I have no other detailed source of the exact composition of SYBR Safe) says this:
SYBR Green I is another dsDNA stain, produced by Invitrogen. It is more expensive, but 25 times more sensitive, and possibly safer than EtBr, though there is no data addressing its mutagenicity or toxicity in humans.
SYBR Safe is a variant of SYBR Green that has been shown to have low enough levels of mutagenicity and toxicity to be deemed nonhazardous waste under U.S. Federal regulations. It has similar sensitivity levels to EtBr,but, like SYBR Green, is significantly more expensive. In countries where safe disposal of hazardous waste is mandatory, the costs of EtBr disposal can easily outstrip the initial price difference, however.
I got from that, that SYBR Safe is 25 times less sensitive than SYBR Green I used in qPCR, also problems with SYBR are that even after all it is an PCR inhibitor and this issue was surely adressed in it's developement for qPCR. Unlike SYBR Safe, which was obviously designed to be less toxic, which led to decreased sensitivity. Probably if more detailed description of the SYBR Safe molecule was available, we could say more, but I'm affraid it's most likely kind of proprietary formula. So there is unknown inhibitory effect of SYBR Safe plus if you wanted to get the same sensitivity you would need to add more of to the reaction, which would definitelly affect the inhition of PCR.
My opinion: I wouldn't use it for qPCR.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
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