Using centrifugal filter units to concentrate protein
Posted 28 February 2013 - 05:26 AM
It doesn't give you a good information on what speeds to use and for how long. Do we just optimise it?
I noticed that solution in the filter would be pulled down even by gravity and a 5 min spin at around 3000rpm would filter out most supernatant while it seems like the manual mentioned a time range between 30-45 min.
Another question is, could you reuse these spin columns?
Posted 28 February 2013 - 08:15 AM
I always had an alternate hypothesis....
Posted 28 February 2013 - 12:07 PM
Posted 28 February 2013 - 03:05 PM
1K as in 1K rpm? or g?
Does the amount of time to take to purify a sample directly correlates to the abundance of the protein of interest in the sample?
Posted 28 February 2013 - 06:36 PM
I reuse them several times but within the same day and for the same sample. You don't want the membrane to dry out between each use either.
Posted 01 March 2013 - 01:06 PM
Posted 01 March 2013 - 02:22 PM
Posted 04 March 2013 - 08:05 AM