Posted 27 February 2013 - 11:16 AM
Posted 27 February 2013 - 11:40 AM
It works via a viral promoter. The true measure of whether or not it is working is to check the protein level - mRNA levels are often but not always correlated to protein levels.
Thank you for the quick and clear answer. Highly appreciated.
Now I understand, it has to be confirmed by quantifying protein levels. But, if I read papers where they have measured mRNA expression using qPCR, it is not completely wrong? Cell machinaries still first transcribe information present in pcDNA to mRNA using viral promoter?
Posted 27 February 2013 - 11:57 AM
Measurement of normal levels of transcript (i.e. untransfected cells) by qPCR is definitely a valid technique, though there are a few problems with that too, as you will find out if you do more of it. All techniques have some problems though, so it is a matter of comparing the problems/difficulties between each and choosing the one that suits you most.
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Posted 27 February 2013 - 12:52 PM
Yes, the cell machinery is ............. All techniques have some problems though, so it is a matter of comparing the problems/difficulties between each and choosing the one that suits you most.
once again my sincere thanks for your insightful reply.