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Freezing DMSO


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#1 Peniel

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Posted 27 February 2013 - 06:34 AM

Is it ok to freeze 1%DMSO and 0.01%DMSO for future use in MTS assay as drug carrier? I know people it at 10% not sure if lower concentration makes a difference.

#2 bob1

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Posted 27 February 2013 - 11:23 AM

1% or less will not prevent the formation of ice crystals, which is what kills the cells. MTS and MTT assays rely on active mitochondria to work - you need your cells to be alive.




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