Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Stromal conditioned medium (SCM)

  • Please log in to reply
1 reply to this topic

#1 yobou



  • Active Members
  • PipPipPipPipPip
  • 93 posts

Posted 27 February 2013 - 12:36 AM

Dear all
can anyone help me know the exact procedures for the production of stromal conditioned medium (SCM) from HS-5 cells. I have been searching the literature but they just mention 50% SCM was used. They do not mention how much percentage of serum was in the medium added to subconfluent cultures of HS-5 cells. also they do not mention what was the final concentration of FBS in the medium with 50% SCM.

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,509 posts

Posted 27 February 2013 - 11:37 AM

I can't talk specifically about stromal conditioned medium, but the general principle is to use the % FBS that you would use for culturing the cells that are going to be in the conditioned medium (i.e. not the HS cells, but the ones you will be growing in the conditioned medium - presumably some sort of stromal cells). If you then add this to the stromal cell medium, which contains the same % FBS then there is no change in concentration.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.