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Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood

TKM1 TKM2 DNA isolation

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#1 srimadh

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Posted 27 February 2013 - 12:32 AM

Hi All,

I want to do DNA isolation form whole blood and I need to make these buffers ... TKM1 and TKM2

Can anyone help me with the recipe for the TKM1 and TKM2 buffers.

Thank you

#2 bob1

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Posted 27 February 2013 - 02:36 PM

TKM :10mM Tris-HCl, pH 7.6, 10 mM KC1, 2 mM EDTA (TKE) containing 4 mM MgCI2 (TKM). No idea about the second one though. It will be a high salt buffer.





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