4 replies to this topic

[asta6801]

Hi

I was wondering if anyone can explain to me how to make buffers? I have very limited chemistry knowledge, so the henderson hasselbach is a bit over my head.

Specifically, I want to make a 150 mM acetate buffer at pH 5.0.

Thanks

[bob1]

Basically what you do is dissolve some sodium or potassium acetate, depending on which salt you want, in water and pH to the desired pH with acetic acid.

[mdfenko]

here is some information to help you understand (and includes buffer tables):

Attached Files

•  Calbiochem_Buffers_Booklet_CB0052_E.pdf   318.75K   59 downloads
•  buffers principles and practice.pdf   513.84K   360 downloads

[asta6801]

Thank you this is very useful stuff!

[neuron]

many buffer calculators are available nowadays. I found this one-

http://www.currentpr...l/toolId-3.html