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Making an acetate buffer

buffer chemistry pH pKa

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4 replies to this topic

#1 asta6801

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Posted 26 February 2013 - 04:15 PM

Hi

I was wondering if anyone can explain to me how to make buffers? I have very limited chemistry knowledge, so the henderson hasselbach is a bit over my head.

Specifically, I want to make a 150 mM acetate buffer at pH 5.0.

Thanks :)

#2 bob1

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Posted 26 February 2013 - 06:45 PM

Basically what you do is dissolve some sodium or potassium acetate, depending on which salt you want, in water and pH to the desired pH with acetic acid.

#3 mdfenko

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Excellent

Posted 27 February 2013 - 07:55 AM

here is some information to help you understand (and includes buffer tables):

Attached Files


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#4 asta6801

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Posted 27 February 2013 - 03:19 PM

Thank you this is very useful stuff!

#5 neuron

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Posted 05 March 2013 - 02:42 AM

many buffer calculators are available nowadays. I found this one-

http://www.currentpr...l/toolId-3.html





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