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SOC vs TB in transformations

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#1 LacquerHead



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Posted 26 February 2013 - 02:24 PM

Hi all, wondering if its absolutely required to use SOC for transforming BL21, XL1 Blue and XL10 Gold cells. I tried it with TB and although I used TB for DH5alpha transformations before with no issues it didnt work here. Any ideas?

Edited by LacquerHead, 26 February 2013 - 02:29 PM.

#2 Trof


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Posted 27 February 2013 - 03:11 AM

SOC is an enriched medium containing glucose, while TB doesn't contain it. Some competent cells may be more sensitive to the initial growing conditions. And as I remember, XL10-Gold cells even require NZY+ broth for transformation (also containing glucose), however when I was doing mutagenesis I didn't have it and used SOC instead and got some colonies. But probably using the right medium would improve the transformation efficiency.

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