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Immunocytohistochemistry & DAPI

DNA DAPI STUFF AHHHHH LPS

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#1 awesomeosaurus

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Posted 25 February 2013 - 01:11 PM

If we activate microglia (induced by LPS). This releases interleukin-1.
We then use immunocytohistochemistry to visualise IL-1.
We also use DAPI which stains DNA. (I THINK it is so we can see the cell death

We have already done a control without LPS ( this image showed a lot more DNA staining and no IL-1 staining)

There is another specific control they are looking for, involving DAPI.

I don't have clue what it is.
Any ideas of ANY type of control, even if it doesn't involve DAPI?

Moved to homework forums. Bob.

#2 bob1

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Posted 25 February 2013 - 02:08 PM

You are correct DAPI does stain DNA... now where do you find DNA and what could you use this to get from a slide that shows discrete spots of DAPI staining?





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