Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Campylobacter transformation


  • Please log in to reply
No replies to this topic

#1 Suziebee

Suziebee

    member

  • Active Members
  • Pip
  • 8 posts
1
Neutral

Posted 25 February 2013 - 09:01 AM

Hi guys,

I was wondering does anyone have a protocol/tips for transforming (electrotransformation) Campylobacter species? They have restriction endonucleases, CRISPR systems and may not recognise plasmids with E. coli ORI's. I've transformed many other bacterial speices with pretty much no problems however Campylobacter is proving very difficult. Posted Image

Thanks in advance for any help/ advice/ tips! Posted Image

All the best,
Susan




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.