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How to measure immunological markers after bacterial infection; IL10-/- Mice

IL10-/- Mice Bacterial infection Inflammation Inflammatory response

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#1 Suziebee

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Posted 25 February 2013 - 08:47 AM

Hi,

Hopefully someone may be able to help me.Posted Image I currently conducting a trial using IL10-/- mice to evaluate the role of an emmerging bacterial gastrointestinal pathogen.
The gross pathology from our prelimary trials look promising, enlarged colon, enlarged spleen and diahorria however I would like to measure the immune response of the mice to this potential pathogen; for instance Thelper1 cells, Tregs, FoxP3.

My poblem is that I do not have access to a flow cytometer so I am hoping somebody may have ideas for an alternative to flow? I was thinking ELISPOT or ELISA but the companies cac't recomend them for tissue homogenate.

I would really appreciate any advice people may have! Posted Image

Thanks in advance,
Susan

#2 bob1

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Posted 25 February 2013 - 11:35 AM

How about Immuofluorescence/immunohistochemistry to get a relative measure of the amount of inflammation and/or number of types of immune cells present in the tissues?

#3 Suziebee

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Posted 26 February 2013 - 07:41 AM

Thank you for your help.




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