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reason of vertical streaks on gels


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#1 Veronika

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Posted 25 February 2013 - 04:41 AM

What are the possible reasons of vertical streaks on gels of 2 DE?

#2 mdfenko

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Posted 25 February 2013 - 06:25 AM

incomplete denaturing of protein spot from first dimension. aggregated protein. too much protein. decomposition. contamination in gel and/or buffers.

above or below (or both) the protein band?

Edited by mdfenko, 25 February 2013 - 06:25 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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