Which is the best way to generate growth curves for cancer cell lines
Posted 25 February 2013 - 02:55 AM
I am quite new in all these processes and would love some ideas.
I want to generate a growth curves for lung cancer cell lines. They are adhering cells. Do I have to always trypsinize them every 12hrs and count them using a haemocytometer?
Do I seed them on 96 well plates and have different plates for 12hrs. 24hrs. 48hrs. 72hrs and so on and then add MTS reagent to read off the absorbance. I am worried about this though because MTS does not stop the growth so they might increase within the 3hr incubation period. I want to be as accurate as possible.
Thanks you all for your kind response.
Posted 25 February 2013 - 04:30 AM
I always had an alternate hypothesis....
Posted 25 February 2013 - 05:22 AM
Posted 25 February 2013 - 11:40 AM
Your comment regarding the MTS assay doesn't make sense - over the course of the assay, at every time point you will have to do the same 3h incubation (and same potential 3 hours of growth), so the measures will still be accurate...
Posted 07 April 2013 - 04:00 AM