Single RE site cloning
Posted 24 February 2013 - 07:42 PM
Trying to clone an insert into a single BamHI site in my vector, wondering what the preferred method is here. I was going to cut the vector with BamHI in the presence of alkaline phosphatase, and then create BamHI sites on both ends of my insert which I will also cut with BamHI after PCR amplification, finally using this cut BamHI sticky end product to ligate into my vector. How does this sound? Any other suggestions?
Posted 24 February 2013 - 08:13 PM
Posted 24 February 2013 - 08:48 PM
Posted 25 February 2013 - 05:12 AM
I always had an alternate hypothesis....