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RNA isolation from Sesame seed

RNA seed

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3 replies to this topic

#1 m_biotech

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Posted 23 February 2013 - 11:27 PM

Hi everybody,

I have problem with RNA isolation from sesame seed as the oil of this plant is so high. Did anybody do it before that can guide me? thanks in advance.

#2 phage434

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Posted 24 February 2013 - 08:40 AM

You should first extract most of the oil with chloroform, then run a normal extraction.

#3 m_biotech

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Posted 25 February 2013 - 10:21 PM

thanks Veteran.

#4 m_biotech

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Posted 02 March 2013 - 10:07 PM

Did anybody have protocol for RNA isolation from oilseed for sesame, rapeseed, etc?




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