Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Determining protein concentration after solubilisation in Triton X-100

bradford protein concentration detergent triton x-100

  • Please log in to reply
2 replies to this topic

#1 Joe101

Joe101

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 21 February 2013 - 03:08 AM

Hi,

The protocol I'm using solubilises brain tissue in 5% Triton X-100 before determining protein concentration buying the Bradford protein assay (for use in SDS-PAGE). I have read however that detergents such Triton X-100 can interfere with the assay; I only need an estimate for gel loading, does anyone know if the presence of this high a level of Tritxon X-100 will significantly effect the results, and if so, suggest any alternatives to the Bradford assay?

Many Thanks,
Joe

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,744 posts
127
Excellent

Posted 21 February 2013 - 06:27 AM

you can try the detergent compatible or reducing agent & detergent compatible bradford methods available from bio-rad.
talent does what it can
genius does what it must
i do what i get paid to do

#3 sam_neuwied

sam_neuwied

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 21 February 2013 - 06:43 AM

You can use BCA protein assay, it is compatible with 5% Triton X-100.

checkout the website
http://www.piercenet...?fldID=02020101





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.