Hi,
The protocol I'm using solubilises brain tissue in 5% Triton X-100 before determining protein concentration buying the Bradford protein assay (for use in SDS-PAGE). I have read however that detergents such Triton X-100 can interfere with the assay; I only need an estimate for gel loading, does anyone know if the presence of this high a level of Tritxon X-100 will significantly effect the results, and if so, suggest any alternatives to the Bradford assay?
Many Thanks,
Joe
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Determining protein concentration after solubilisation in Triton X-100
Started by Joe101, Feb 21 2013 03:08 AM
bradford protein concentration detergent triton x-100
2 replies to this topic
#2
mdfenko
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an elder
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Excellent
Posted 21 February 2013 - 06:27 AM
you can try the detergent compatible or reducing agent & detergent compatible bradford methods available from bio-rad.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
sam_neuwied
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Posted 21 February 2013 - 06:43 AM
You can use BCA protein assay, it is compatible with 5% Triton X-100.
checkout the website
http://www.piercenet...?fldID=02020101
checkout the website
http://www.piercenet...?fldID=02020101
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