Dear All,
Sorry, is me again.
I would like to ask for suggestion if it is better to extract the RNA from whole pancreas or from islets of a mice to do the RT-PCR for gene expression study?
Thank you very much.
Submit your paper to J Biol Methods today!
Gene expression from whole pancreas or islets by using RT-PCR
Started by wdyeo, Feb 21 2013 03:04 AM
2 replies to this topic
#2
pcrman
-
- Global Moderators
-
- 1,165 posts
Epigenetist
69
Excellent
Excellent
Posted 21 February 2013 - 12:03 PM
It depends on the purpose of your study. If you want to look for expression specifically in islets and you can isolate islets, why not just using islets for RNA isolation?
#3
wdyeo
-
- Active Members
-
- 54 posts
Enthusiast
4
Neutral
Neutral
Posted 23 February 2013 - 11:42 AM
Hi Epigenetist,
Yeah, indeed is better to use islets..
But it is kinda troublesome to isolate RNA from islets than pancreas. I am wondering will there be great difference of the gene expression using islets or pancreas?
Thank you.
Yeah, indeed is better to use islets..
But it is kinda troublesome to isolate RNA from islets than pancreas. I am wondering will there be great difference of the gene expression using islets or pancreas?
Thank you.
