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Gene expression from whole pancreas or islets by using RT-PCR


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#1 wdyeo

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Posted 21 February 2013 - 03:04 AM

Dear All,
Sorry, is me again.
I would like to ask for suggestion if it is better to extract the RNA from whole pancreas or from islets of a mice to do the RT-PCR for gene expression study?
Thank you very much.

#2 pcrman

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Posted 21 February 2013 - 12:03 PM

It depends on the purpose of your study. If you want to look for expression specifically in islets and you can isolate islets, why not just using islets for RNA isolation?

#3 wdyeo

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Posted 23 February 2013 - 11:42 AM

Hi Epigenetist,
Yeah, indeed is better to use islets..
But it is kinda troublesome to isolate RNA from islets than pancreas. I am wondering will there be great difference of the gene expression using islets or pancreas?
Thank you.




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