Gene expression from whole pancreas or islets by using RT-PCR
Posted 21 February 2013 - 03:04 AM
Sorry, is me again.
I would like to ask for suggestion if it is better to extract the RNA from whole pancreas or from islets of a mice to do the RT-PCR for gene expression study?
Thank you very much.
Posted 21 February 2013 - 12:03 PM
Posted 23 February 2013 - 11:42 AM
Yeah, indeed is better to use islets..
But it is kinda troublesome to isolate RNA from islets than pancreas. I am wondering will there be great difference of the gene expression using islets or pancreas?