Hi everyone!
I would like to ask if anyone has any experience in electrophoresis and detection of hyaluronate. I did PAGE of a digest mixture (consisting of HA chains of various lengths) according to numerous protocols (15% and 10% gel in TBE, linear gradient 20%-3% and 18%-1,5%, with samples in NaOAc, NH4HCO3, NaOAc+HCl, dH20
or TBE, with the voltage of 300V and 100V and detection by Alcian Blue in water, water+2% acetic acid or water+40%methanol+10% acetic acid.
Everytime I just got an ugly smudge instead of nice "ladder". Today I will try Alcian Blue+Silver staining protocol, but I doubt whether it can do any good. I think a smudge is a smudge, no matter how you dye it, though I still hope I am wrong.
Does anyone have any idea what else I could try?
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Hyaluronate electophoresis
Started by EvilTwin, Feb 21 2013 01:04 AM
PAGE electrophoresis polysaccharide hyaluronate uronic acid
1 reply to this topic
#2
mdfenko
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an elder
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Excellent
Excellent
Posted 21 February 2013 - 06:14 AM
you are correct, a smudge is a smudge.
you can try the procedure in this paper:
microanalysis of ...
it contains page and references to ce methods.
or you can see this paper for ce methods:
ce for analysis of ...
you can try the procedure in this paper:
microanalysis of ...
it contains page and references to ce methods.
or you can see this paper for ce methods:
ce for analysis of ...
Edited by mdfenko, 21 February 2013 - 06:15 AM.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
