Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

cytoplasmic and nuclear extraction

  • Please log in to reply
No replies to this topic

#1 silvepa23



  • Active Members
  • Pip
  • 21 posts

Posted 20 February 2013 - 10:20 PM


i have been using the NE-PER Nuclear and Cytoplasmic Extraction Reagents by thermo scientific Pierce. When I run both the nuclear (15 ug) and cytoplasmic (40 ug) extracts I use b-actin as a control. When I expose the membrane I get very strong signal in the nuclear extracts and weaker signal in the cytoplasmic despite the fact that i added more than 2x the cytoplasmic protein to the gel and there should be more in the cytoplasm. has anyone else used this kit and had a similar problem. thanks

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.