Edited by neuron, 18 February 2013 - 11:17 PM.
Confirmation of ligationPCR RD
Posted 18 February 2013 - 11:16 PM
Posted 18 February 2013 - 11:45 PM
A good strategy is to either use sequencing primers for your plasmid or to use one primer anchored in the insert and one in the plasmid.
Posted 19 February 2013 - 12:45 AM
Posted 19 February 2013 - 02:16 AM
I always had an alternate hypothesis....
Posted 19 February 2013 - 03:07 AM
Posted 19 February 2013 - 07:36 AM
Edit: I think you mentioned that you can not see a difference between your linear vector and your vector + insert on a gel? If I were you, I would run a very low % gel so I could isolate the ligated vector + insert band. It will save you loads of time during screening of your clone.
Edited by jerryshelly1, 19 February 2013 - 07:38 AM.
Posted 20 February 2013 - 08:04 PM