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Can I activate signaling pathways without extracellular stimuli?

signaling pathways MAPK cell culture

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4 replies to this topic

#1 MariaGreen

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Posted 16 February 2013 - 02:19 PM

For example the MAPK pathway in Hela cells or SW480? or any other cancer cell line for that matter.

#2 bob1

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Posted 16 February 2013 - 11:42 PM

Not as far as I know - you need some sort of stimulus, and that can only come from outside if you want to get a lot of cells all doing the same thing at the same time.

#3 MariaGreen

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Posted 17 February 2013 - 03:47 AM

Okey, in some way it does not add up to me. If we have cancer cells and the pathways are disrupted in them in some way, meaning that the pathways are constitutively acive, In that case, we should be abla to detect activation without stimulation, at least compared to healthy cells.

Tank you for your answer

#4 bob1

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Posted 17 February 2013 - 11:27 AM

Yes, in that case you, if the pathways are constituitvely activated, you should be able to see it - but that isn't what you asked!

#5 MariaGreen

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Posted 17 February 2013 - 11:43 AM

Fair enough, that was not really my question to begin with. Thank you for your time.





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