PCR and restriction enzyme digestion
Posted 16 February 2013 - 03:45 AM
Posted 16 February 2013 - 11:47 PM
Posted 17 February 2013 - 12:25 AM
Posted 17 February 2013 - 11:30 AM
Keep this in mind - a small fragment of DNA cut out of a plasmid may not show up on a gel if there isn't enough DNA to bind the EtBr and give a visible band. So, 200 ng of plasmid may not be enough to see a cut out band, though you should be able to see the initial plasmid band.