Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Difference between 2% PFA and 4% PFA


  • Please log in to reply
3 replies to this topic

#1 science noob

science noob

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 279 posts
20
Excellent

Posted 15 February 2013 - 04:32 AM

Any immunocytochemistry experts out there who can clarify what exactly does PFA do when used as a fixative? Why are there two preferred concentrations which people use when fixing cells/tissue?

Also, the fixation times vary from protocol to protocol. Some recommend 10-15 min, some more than 30 min. What will over-fixing do to the cells?

#2 anamaria

anamaria

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 15 February 2013 - 11:52 AM

we are using PFA 4% for brain tissue for maximum 30 min. Usually between 10-30min should be ok

#3 anamaria

anamaria

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 15 February 2013 - 11:56 AM

over-fixing of tissue can inactivate some antigens

#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,728 posts
399
Excellent

Posted 15 February 2013 - 10:50 PM

Check out this reference: Fox et al., The Journal of Histochemistry and Cytochemistry Vol. 33, No. 8, pp. 845-853, 1985




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.