Any immunocytochemistry experts out there who can clarify what exactly does PFA do when used as a fixative? Why are there two preferred concentrations which people use when fixing cells/tissue?
Also, the fixation times vary from protocol to protocol. Some recommend 10-15 min, some more than 30 min. What will over-fixing do to the cells?
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#2
anamaria
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Posted 15 February 2013 - 11:52 AM
we are using PFA 4% for brain tissue for maximum 30 min. Usually between 10-30min should be ok
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anamaria
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Posted 15 February 2013 - 11:56 AM
over-fixing of tissue can inactivate some antigens
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bob1
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Posted 15 February 2013 - 10:50 PM
Check out this reference: Fox et al., The Journal of Histochemistry and Cytochemistry Vol. 33, No. 8, pp. 845-853, 1985
