Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Sodium orthovanadate (Na2VO3) preparation


  • Please log in to reply
3 replies to this topic

#1 science noob

science noob

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 272 posts
20
Excellent

Posted 15 February 2013 - 04:30 AM

Anyone prepared Na3VO4 to be used as a component of the protein lysis buffer before?

It seems like the pH has to be readjusted to pH8 using a tricky protocol.

And I heard you would have to activate it prior to usage by boiling?

Edited by science noob, 15 February 2013 - 07:05 AM.


#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,703 posts
123
Excellent

Posted 15 February 2013 - 05:02 AM

do you mean na3vo4?

see this webpage:

sodium orthovanadate activation

Edited by mdfenko, 15 February 2013 - 05:02 AM.

talent does what it can
genius does what it must
i do what i get paid to do

#3 science noob

science noob

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 272 posts
20
Excellent

Posted 15 February 2013 - 05:52 AM

do you mean na3vo4?

see this webpage:

sodium orthovanadate activation


Do you have to reactivate it after thawing out frozen aliquots?

I found that some aliquots appear liquid at -20C and dont freeze. Would you know what is it so?

Edited by science noob, 15 February 2013 - 05:53 AM.


#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,703 posts
123
Excellent

Posted 19 February 2013 - 12:43 PM

Do you have to reactivate it after thawing out frozen aliquots?

check the pH, if it's low then yes, but, since the activation procedure says to store aliquots of active orthovanadate at -20C, you probably won't have to.

I found that some aliquots appear liquid at -20C and dont freeze. Would you know what is it so?

not a clue (maybe it's concentrated enough to suppress the freezing point to lower than -20C, maybe your freezer is off, maybe you looked during defrost cycle?)
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.