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finding gene sequence to delete


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4 replies to this topic

#1 lmg

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Posted 14 February 2013 - 11:00 PM

Please if you can help.

My task is to find/locate a gene for oxoglutarate dehydrogenase (ketoglutarat dehydrogenase) in Streptomyces lividans or in Streptomyces coelicolor. I need a nucleotide sequence in order to delete it.

I have worked in human genetic lab till now and I am not familiar with this bacterial genome at all... Which page to use (I am used to ensembl for humans) to get a gene nt seq and locate it in bacterial genome?

Please help....

#2 christy

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Posted 14 February 2013 - 11:27 PM

Hi,
Here is a link.

http://www.genome.jp...ry=Streptomyces


In this link there is no Streptomyces lividans, but if the sequence is similar to the other streptomyces species in this link you can use that. I am working with E. coli. Usually we use different E. coli sequences. Mostly same genus carries almost similar sequences. I found your ketoglutarate dehydrogenase in Streptomyces coelicolor, 1272 aminoacids.

Best of luck,

#3 neuron

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Posted 14 February 2013 - 11:32 PM

http://www.genedb.or...age/Scoelicolor

http://www.xbase.ac....s-lividans-tk24

#4 lmg

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Posted 15 February 2013 - 12:57 AM

Thanks Christy! You are my savior!

lmg

#5 lmg

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Posted 15 February 2013 - 12:58 AM

thanx neuron. ;-)

lmg




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