4 replies to this topic

[lmg]

Please if you can help.

My task is to find/locate a gene for oxoglutarate dehydrogenase (ketoglutarat dehydrogenase) in Streptomyces lividans or in Streptomyces coelicolor. I need a nucleotide sequence in order to delete it.

I have worked in human genetic lab till now and I am not familiar with this bacterial genome at all... Which page to use (I am used to ensembl for humans) to get a gene nt seq and locate it in  bacterial genome?

Please help....

[christy]

Hi,
Here is a link.

http://www.genome.jp...ry=Streptomyces


In this link there is no Streptomyces lividans, but if the sequence is similar to the other streptomyces species in this link you can use that. I am working with E. coli. Usually we use different E. coli sequences. Mostly same genus carries almost similar sequences. I found your ketoglutarate dehydrogenase in Streptomyces coelicolor, 1272 aminoacids.

Best of luck,

[neuron]

http://www.genedb.or...age/Scoelicolor

http://www.xbase.ac....s-lividans-tk24

[lmg]

Thanks Christy! You are my savior!

lmg

[lmg]

thanx neuron. ;-)

lmg