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Staining lysed cells in Drosophila

lysed drosophila phage virus

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#1 levuccio



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Posted 14 February 2013 - 02:35 PM


I am interested in looking at a virus in Drosophila which overeplicates, and then lyses the cells to infect new ones.
I would like to be able to detect the lysis in adult drosophila flies, to look at the timing of lysis. Is there any way that I can
stain only the cells that have been lysed by the virus? I know there is Acridine orange which stains apoptotic cells,
however apoptosis and phage lysis are a bit different to each other.

Does anyone have any ideas? Is there any other method?



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