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Integrin B1 Staining in HT1080 cells

integrin B1 staining IF antibody adhesions

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#1 Elma

Elma

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Posted 14 February 2013 - 09:56 AM

Hi everyone,

I am looking at total amount of B1 integrins in HT1080 cells using TIRF microscopy. I used the Huts-4 antibody for active B1 and got good localization at adhesions. However, I have used 2 other antibody to look at the total B1 integrin levels and I see nothing specific... No localization at adhesions... So, if you know of any good antibody for detecting total level of B1 integrin, please sharePosted Image . And if you have encountered similar problem, also please share suggestions/advice. Thanks!

I am fixing with 2% PFA (0.12 M sucrose), and permebealizing with 0.2% triton X


Best,
Elma

#2 Curtis

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Posted 14 February 2013 - 11:42 PM

you can check which region they bind to at company websites. they must indicate amino acid numbers. so chose antibodies that bind to where Huts4 binds to. I do understand it is active form of B1, and this region might be hidden inside in inactive form, but still check the binding regions.





Also tagged with one or more of these keywords: integrin B1, staining, IF, antibody, adhesions

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