I am currently extracting RNA from circulating PBMCs.
My current experiment requires that I extract RNA at 10 minute intervals.
As it takes around 30 minutes for me to extract RNA (Qiagen RNeasy), it is clear that I really can't perform my experiment without some help.
I was wondering whether anyone had used buffer RLT to disrupt their cells, and then placed them on ice until a time when the rest of the RNA extraction protocol could be followed?
If not, then any other suggestions would be most welcome.
Many thanks in advance.
Edited by rumjon, 13 February 2013 - 07:44 AM.