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PBMC lysis protocol?


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#1 suisseju

suisseju

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Posted 13 February 2013 - 03:21 AM

hi
does anybody have a protocol for or experience in lysis of PBMC? I would like to use the proteins in an immunoassay. Is it possible to lyse it by sonication? Do I need a special lysis buffer?
thanks for your help.
best regards

Edited by suisseju, 13 February 2013 - 04:56 AM.


#2 Curtis

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Posted 13 February 2013 - 09:44 PM

most lyssis buffers will do. make sure you check the main page of protocol-online.org. there are plenty of protocols and recipes. If you still have questions come back to the forum and ask.




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