Tried with the Promega Wizard kit, has worked fine for plant tissue and bacterial cells from past experience, but not mycelia (in all cases material was first ground using mortar/pestle & liquid nitrogen). Then I tried something I thought should work (using proteinase K) but that was even worse for getting DNA. My first step was to use RNase A at 37 C in buffer of 150 mM tris, 25 mM EDTA, and 1% SDS, but that didn't seem to work at all (and this was before proK). Nanodrop data for all was quite good in terms of nucleic acid purity; on the gel I loaded 200-250 ng of each sample.
I don't know why I am getting such terrible results, both ways. Anyone get good DNA from fungus & how do you do it? I'm working with the Diaporthe sp.
and Phomopsis sp.
, btw, which are both Ascomycota.