I did WB against histone H3 .. Antibodies are from Abcam .. Anti H3 blot was done against total histones from Chicken. Now, eventhough the antibody does pick some H1 as well but in my blot i found my blot to detect a band upper to H3 in addition to H3 and delta H3 .
Can anybody pls help me with that ?
EPIGENETICS : Help required ... Histone Western Blot
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