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acceptable melt curve?

Started by joy123, Feb 12 2013 07:36 AM

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#1 joy123

joy123

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Posted 12 February 2013 - 07:36 AM

Hi I am doing qPCR. I wonder if you could help me judge if these melt cuve are good enough? I don't understand why there is a shoulder at ~74C and 70C. Is this set of primer specific enough for qPCR?

Thanks a lot!

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  • Ra primer A melt curve.jpg

#2 joy123

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Posted 14 February 2013 - 08:36 AM

Hi! Can anybody please help me with this problem? Thanks!
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