Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Validation of Up-regulated genes

Started by mhj222, Feb 11 2013 06:48 AM

Gene expression

Please log in to reply

1 reply to this topic

#1 mhj222

member

Active Members
6 posts

0
Neutral

Posted 11 February 2013 - 06:48 AM

I can use siRNA to knock down and validate the effects of genes that are down regulated. How do you validate genes that are up-regulated? I have been thinking of using SINEUP which can increase expression of targeted endogenous genes. Does anyone have any experience with this or tried alternatives?

#2 pcrman

Epigenetist

Global Moderators
1,021 posts

43
Excellent

Posted 11 February 2013 - 08:44 AM

Traditionally, overexpression of a gene is achieved by vector-based systems which introduce an exogenous gene.

BTW, what is SINEUP? I did not find anything on google and pubmed.

Back to Macroarray and Microarray · Next Unread Topic →

Also tagged with one or more of these keywords: Gene expression

Protocols and Techniques Forums → Tissue and Cell Culture → Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any Started by Guest_It'sNotMe_* , 08 May 2013 Gene Expression	3 replies 102 views	bob1 09 May 2013
Protocols and Techniques Forums → Bioinformatics and Biostatistics → Gene expression database Started by Guest_LauradU_* , 31 Jan 2013 gene expression	1 reply 237 views	pcrman 31 Jan 2013
Protocols and Techniques Forums → Macroarray and Microarray → How to interpret RES file format to calculate expression data Started by Guest_monukumar_* , 03 Oct 2012 res, microarray, gene expression	0 replies 403 views	monukumar 03 Oct 2012
Protocols and Techniques Forums → Macroarray and Microarray → How to present microarray data of two samples? Started by Guest_kaveh_* , 09 May 2012 microarray, microRNA and 1 more...	0 replies 518 views	kaveh 09 May 2012
Protocols and Techniques Forums → Protein and Proteomics → Expression level analysis at mRNA vs protein level Started by Guest_biochemical mayhem_* , 09 Apr 2012 gene expression	6 replies 643 views	biochemical mayhem 11 Apr 2012