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Validation of Up-regulated genes

Started by mhj222, Feb 11 2013 06:48 AM

Gene expression

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#1 mhj222

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Posted 11 February 2013 - 06:48 AM

I can use siRNA to knock down and validate the effects of genes that are down regulated. How do you validate genes that are up-regulated? I have been thinking of using SINEUP which can increase expression of targeted endogenous genes. Does anyone have any experience with this or tried alternatives?

#2 pcrman

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Posted 11 February 2013 - 08:44 AM

Traditionally, overexpression of a gene is achieved by vector-based systems which introduce an exogenous gene.

BTW, what is SINEUP? I did not find anything on google and pubmed.

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