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I am a freshman in WB. Could anyone recommend good commercial antibodies for His-tag and StrepII-tag? Thanks a lot!
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Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies?
Started by nanopore, Feb 07 2013 10:43 PM
5 replies to this topic
#2
Curtis
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Posted 07 February 2013 - 10:50 PM
I am going to tag my protein with His but I want to purify without anti-His. They told me to use nickel columns. check Pierce for kits, or Abcam for antibodies.
#3
nanopore
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Posted 07 February 2013 - 10:53 PM
Hi, So you mean Abcam's antibodies are good?
Curtis, on 07 February 2013 - 10:50 PM, said:
I am going to tag my protein with His but I want to purify without anti-His. They told me to use nickel columns. check Pierce for kits, or Abcam for antibodies.
#4
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Posted 07 February 2013 - 11:02 PM
that is like saying if Burger King sells burgers?
#5
Missle
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Posted 14 February 2013 - 10:13 AM
I use Maine Biotechnology Services anti-His MAb (MAb 230P - I think) and it works quite well for western blot and ELISA purposes.
#6
PhDinAcronyms
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Posted 14 February 2013 - 04:01 PM
Qiagen also has a variety of anti-his antibodies that can be specific to your his tag if you don't have a simple 6xhis that is easily detected (5xhis, 4xhis etc) and they also have nickel agarose for purification. You can buy it all as a kit
