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Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies?


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#1 nanopore

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Posted 07 February 2013 - 10:43 PM

This topics may be already talked for many times...

I am a freshman in WB. Could anyone recommend good commercial antibodies for His-tag and StrepII-tag? Thanks a lot!

#2 Curtis

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Posted 07 February 2013 - 10:50 PM

I am going to tag my protein with His but I want to purify without anti-His. They told me to use nickel columns. check Pierce for kits, or Abcam for antibodies.

#3 nanopore

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Posted 07 February 2013 - 10:53 PM

Hi, So you mean Abcam's antibodies are good?

I am going to tag my protein with His but I want to purify without anti-His. They told me to use nickel columns. check Pierce for kits, or Abcam for antibodies.



#4 Curtis

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Posted 07 February 2013 - 11:02 PM

that is like saying if Burger King sells burgers?

#5 Missle

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Posted 14 February 2013 - 10:13 AM

I use Maine Biotechnology Services anti-His MAb (MAb 230P - I think) and it works quite well for western blot and ELISA purposes.

#6 PhDinAcronyms

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Posted 14 February 2013 - 04:01 PM

Qiagen also has a variety of anti-his antibodies that can be specific to your his tag if you don't have a simple 6xhis that is easily detected (5xhis, 4xhis etc) and they also have nickel agarose for purification. You can buy it all as a kit




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