Ct value very high. What can I do to improve this ct?
Posted 07 February 2013 - 07:26 AM
I work with Sybr to analyze 5-HT (Serotonin receptor) in rat hypothalamus. However, during the primer optimization the ct value for this gene has been very high (30- 32). We have already redesigned twice the primers for 5-HT and we got same result. Last experiment, we concentrated the cDNA pool samples for 1:5 and the ct didn't change.
I don't know what to do in this case.
Can anybody help me?
Thanks a lot
Posted 07 February 2013 - 08:44 AM
Posted 07 February 2013 - 09:51 AM
Posted 07 February 2013 - 11:00 AM
Posted 07 February 2013 - 11:04 AM
I have used 1ug of RNA for RT and my primer is specific for the gene.