Posted 07 February 2013 - 01:43 AM
The data sheet you attached says: "These Primers and Probe are synthesised and purified by PAGE. They are supplied as 0.2 OD (6.6 μgs) lyophilized. To reconstitute, dissolve in 20 μl sterile water, check concentraion by OD before use."
So, according to this you just have to add 20µl of water. The predicted concentration will be 330ng/µl (6.6µg/20µl), but you should confirm by nanodrop or similar methods. From this dilute to your working concentration.
I'm not sure where you get that "The lyophilized concentration of primer is 20 pmol" On that, a very important point. 20pmol is NOT a concentration, is total amount. Same goes for 7µg (or actually 6.6µg according to the attached document), this is NOT a concentration but an absolute amount. You can make this up to the required concentrations by adding water (or TE).
Back to what the data sheet suggests, of dissolving in 20µl of water, and assuming that 6.6µg of primers are indeed 20pmol. Primers dissolved this way will be at a concentration of 1µM (20pmol/20µl).
Hope this helps.