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Adipogenic differentiation and senescence marker


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#1 Johndg2

Johndg2

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Posted 06 February 2013 - 04:04 PM

Hello, I'm wondering how long I need to differentiate (adipogenic) my cells so that I can do qPCR and Western for senescence markers. I'm on day 5 of adipogenic differentiation and I was thinking of extracting RNA and protein tomorrow on Day 6. When are secencent markers (p21 etc.) expressed in differentiating adipocytes? Thanks in advance!




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