Amount of RNA (ng) for good ct values.
Posted 06 February 2013 - 10:34 AM
Posted 17 February 2013 - 11:23 PM
Sorry for the late response.
Hmmm.. I don't really know the kit I am using to make cDNA. All the reagents I use were taken out of the package. I remember I use random hexamers and denature the RNA then add rnase, dntps, a buffer, and ssi (i think that's the name).
Posted 18 February 2013 - 08:38 AM